Hi everyone,
This might be a very simple question.
I’m following this microarray workflow- www.semel.ucla.edu/sites/default/files/coppola-lab/CoppolaExampleCode.pdf.
These are the R codes I have done so far.
Microarray.MSCs <- read.delim(“/Volumes/EDEN/phd/Microarray MSCs.txt”, header=FALSE)
dim(Microarray.MSCs)
[1] 32408 14
head(Microarray.MSCs)
V1 V2 V3 V4 V5 V6 V7 V8 V9
1 ProbeName Day 0-1 Day 0-2 Day 0-3 Day 7-1 Day 7-2 Day 7-3 Day 14-1 Day 14-2
2 A_04_P063001 666.636 1696.1885 1557.941 6252.668 2919.4412 5536.268 12564.507 10459.793
3 A_04_P027222 134.40279 163.9967 150.52025 1936.827 583.9774 1742.507 1738.8904 1738.4939
4 A_04_P054052 79.53051 149.24582 276.84015 351.02386 332.94275 482.743 406.8957 289.9612
5 A_04_P043272 4.9999995 7.891682 20.59979 66.30415 57.350246 65.05819 46.807858 47.90688
6 A_04_P082033 4.9999995 5.562969 22.03437 28.541346 21.72589 45.44614 22.657038 9.8729105
V10 V11 V12 V13 V14
1 Day 14-3 Day 21-1 Day 21-2 Day 21-3 GeneSymbol
2 10751.576 7159.59 5940.33 6241.056 LOC100346751
3 1691.0352 899.52216 653.9722 702.0644
4 411.41324 154.12201 126.39794 397.99387
5 45.024284 29.569155 26.168947 69.53682 LOC100342109
6 16.986622 7.7133107 4.9999995 57.2613
It says that I now need to read in the sample info on page 2. This is stored in the targets.txt file however I only have one file of data, an excel spreadsheet that I converted into a txt file.
Does anyone know what this means or how I might make one.
Also does anyone know how to remove the non-numeric-alike variable(s) in data frame: V2, V3, V4, V5, V6, V7, V8, V9, V10, V11, V12, V13.
Thankyou!
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