BCL files conversion to FASTQ without SampleSheet.csv

BCL files conversion to FASTQ without SampleSheet.csv

1

Dear community,

I have got NGS data which is basically the BaseCalls folder with .bcl files. I want to know how to successfully convert .bcl files to .fastq format. So far, I have been using the bcl2fastq program, however, I have no SampleSheet.csv file. I have generated my own file like this because the program crashes otherwise.

My own generated SampleSheet.csv file

Is it a good way to do it? Am I missing something? Unfortunately, I have not been working with .btl data so far. Also, I have not done sequencing myself.

By the way, now bcl2fastq doest not crash but spamming this message:

Running program


fastq


demultiplexing


bcl2fq-local


bcl

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