miRDeep2 whitespaces error
Hi everyone,
I am trying to run miRDeep2.pl and I keep getting the following error message:
#Starting miRDeep2
[1;31mError: [0mmiRNA reference this species file miRBase_ame.fa has not allowed whitespaces in its first identifier
As far as I can tell, it doesn’t have any white spaces in its first identifier.
The first line of the file looks like this
>ame_miR_3736
I’ve checked manually by opening in notepad and there isn’t a whitespace hanging around at the end of the line – I also ran a script to remove any just in case.
It looks exactly the same as the .fa file given in the miRDeep2 tutorial
>cel-miR-36
except that I have underscores instead of hyphens. If I change it to hyphens I get exactly the same error message.
Any suggestions?
If I don’t input a known miRNA ref file it runs OK.
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