miRDeep2 whitespaces error

miRDeep2 whitespaces error

0

Hi everyone,

I am trying to run miRDeep2.pl and I keep getting the following error message:

#Starting miRDeep2
[1;31mError: [0mmiRNA reference this species file miRBase_ame.fa has not allowed whitespaces in its first identifier

As far as I can tell, it doesn’t have any white spaces in its first identifier.
The first line of the file looks like this

>ame_miR_3736

I’ve checked manually by opening in notepad and there isn’t a whitespace hanging around at the end of the line – I also ran a script to remove any just in case.
It looks exactly the same as the .fa file given in the miRDeep2 tutorial

>cel-miR-36

except that I have underscores instead of hyphens. If I change it to hyphens I get exactly the same error message.

Any suggestions?

If I don’t input a known miRNA ref file it runs OK.


next-gen


miRDeep2

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