Picard demultiplexing with random barcodes
I am demultiplexing RNA-Seq data using Picard. I normally fill out the
BARCODE_1 for the sample barcodes and
BARCODE_2 for the UMI barcodes.
However, in this run, I don’t know the UMI barcodes. They came from a pool of random barcodes and are 10nt in length.
Now I don’t know how to fill out the
BARCODE_2 row in my library files. Leaving it empty, filling it with “N” or “NNNNNNNNNN” lead to errors like
Row for barcode null appears more than once in LIBRARY_PARAMS or BARCODE_PARAMS file.
Has anyone worked with this?
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