How to remove introns in a specific nucleotide sequence
I am using ribotricer software to search for ORF in ribo-seq data. This software will return the start and end positions of the ORF on the genome as below:
ENST00000327044.7_944697_959240_2247 chr1 –
The information in the above line is, in order, gene information, gene start position, gene end position, ORF length, chromosome, positive and negative chain.
I want to use it to predict the amino acid sequence, but I found that the nucleotide sequence obtained in this way has introns. How can I pass it? Use annotation files to remove introns in such a sequence?
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