RNA-Seq by MinION

RNA-Seq by MinION

0

Hi all,

I would like to use MinION for RNA-seq analysis for more than 20 samples of baker’s yeast. I am wondering if anybody has ever tried barcoding kit to do multiplexing RNA-Seq? The output per flow cell is between 7-12 M reads in general, and if I use maximum 12 barcoding capacity of barkoding kit per flow cell on MinION, around 1 M per sample is generated. But, it is unclear to me whether this read depth is enough for differential gene expression analysis as it generates long reads, while Ilumina reads are short, but 5 to 10 M reads and more are generally generated. Any suggestions would greatly help me in making decision.

Thank you.


dge


MinIon


rna-seq

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