cellranger count output does not give all genes.

cellranger count output does not give all genes.


Dear all,

I have recently started using the cellranger from 10x for scRNA-seq data, after having used my own pipeline (with STAR alignment) for smart-seq2 data, to get the count matrix for then later analyzing with Seurat or Scanpy.

I have however faced a strange issue: not all genes appear in the features.csv file.
For example, the gene Clec7a does not appear, which seems strange to me.
I then checked the total number of features present in the features.csv file and it was approximately 32000, which is very different from the 53000 I used to have with my own pipeline. This seems to indicate that the genome annotation files (gtf) are somehow different.

Does 10x filter the genome annotation file in a certain way to decrease the number of genes? Is there an option to control this?

Note: I use the same reference genome version in my own pipeline as the 10x one.





Read more here: Source link