How to define gold standard protein interaction? How to construct such a gold standard dataset?

How to define gold standard protein interaction? How to construct such a gold standard dataset?

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To my knowledge, protein interactions identified in experiments are usually compared with a reference dataset that is considered as gold standard. And that the gold standard interaction dataset is usually considered as containing true positive interactions only. But I want to know how do we create such a gold standard dataset at the first place?


interaction


protein

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