Metagenomics normalization for long reads
Hello everyone,
I am currently working on a metagenomics analysis using ONT and I have used epi2me wimp for classification of the reads. What I want is to plot the relative abundance of each species present in the sample.
Epi2me outputs a read count assigned to each taxa so I can produce a plot for the proportion of reads per taxa but since each reads has a different length should I normalize by read length ?
• 34 views
Read more here: Source link