Postdoc Position in non-coding RNA & R-loop Epigenetics at the Institute of Molecular Biology in Mainz, Germany

The Niehrs laboratory studies regulation of DNA methylation, which plays important roles in development & disease. We have recently demonstrated a role for long non-coding RNAs and R-loop DNA:RNA hybrids in DNA methylation (Arab et al. 2014; Arab et al. 2019). In an ERC-funded project, we now aim to elucidate the mechanisms of DNA demethylation as well as the role played by long non-coding RNAs and R-loops in its regulation. See also

The project

Candidates will make use of mouse embryonic stem cells (ESCs) to interrogate genome-wide the role of ncRNAs and R-loops in targeting GADD45a and the DNA demethylation machinery in the genome. We will use CRISPR/CAS9 mediated ESC mutants, as well as ChIP-seq, RNA-seq, bisulfite-seq to carry out multi- dimensional data analysis to address: Where in the genome does demethylation occur? What are the molecular determinants in ncRNA & Rloops involved in targeting? What are the cofactors involved? The project will involve strong interaction between the Molecular- and the Computational Biologist.

Requirements of the candidate

• PhD & publications in a relevant field

• Sound experience in molecular biology

• Familiarity with Omics techniques or –analysis, or with ncRNAs or R-loops is advantageous

• Excellent communication skills and good team spirit with the ability to solve problems independently

• Fluent in English (spoken and written)


Arab, A. et al. (2019) GADD45A binds R-loops and recruits TET1 to CpG island promoters Nature Genetics 2:217-223.

Schäfer A, et al. C (2018). Impaired DNA demethylation of C/EBP sites causes premature aging. Genes Dev. 32:742-762

Arab, K., et al. (2014) Long Noncoding RNA TARID Directs Demethylation and Activation of the Tumor Suppressor TCF21 via GADD45A. Mol. Cell. 55, 604-614

Niehrs, C. and Schäfer, A. (2012) Active DNA demethylation by Gadd45 and DNA repair. Trends Cell Biol. 22, 220-227

Barreto, G., Schäfer, A., Marhold, J., Stach, D., Swaminathan, S.K., Handa, V., Döderlein, G., Maltry, N., Wu, W., Lyko, F. and Niehrs, C. (2007) Gadd45a promotes epigenetic gene activation by repair-mediated DNA demethylation. Nature 445, 671-675

How to apply

Please see details on the project, information on how to apply, and an online application form at IMB is an equal opportunity employer.

Starting date: 1 April 2022 or later. The deadline for applications is 25 November 2021. Interviews will take place in Mainz on 20-21 January 2022

Application webpage: []

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