Should you normalization metagenomic data
I am given to understand you should not normalize when you have a smoother coverage and expected amount of data base on BBNorms documentation. Does this mean coverage over the resulting assembly or over something else? However ,given that normalisation is often used to make a data set computationally tractable I am sure my understanding must be incorrect?
Furthermore I have been informed that metagenomic data tends to have a more smooth coverage so then, should you ever normalize metagenomic data?
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