Is it possible for a single DNA nucleotide to match its cDNA complement, and not invert?
This is likely a simple biology question but I am having trouble understanding. I am reading about a single nucleotide mutation, with the mutation in the cDNA described as: RDH12:c.505C>G.
However, the gDNA change is similarly described as chr14:g.68193754C>G.
I checked the reference sequence, and at this position the reference nucleotide is indeed C.
How is it possible for the cDNA at that position to also have C? Shouldn’t it be the reverse complement?
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