3′ enrichment vs full-length sequencing
I’m new to this community (and also to this field in some sense) and I have a question.
In the paper “Luecken MD, Theis FJ. Current best practices in single-cell RNA-seq analysis: a tutorial. Mol Syst Biol. 2019 Jun 19; 15 (6): e8746. Doi: 10.15252 / msb.20188746. PMID: 31217225 ; PMCID: PMC6582955. ” on page 5 it says that sequencing techniques can be divided into 3 ‘enrichment or full-length, and depending on the method, a certain normalization technique is recommended.
I would like to know how I could deduce the sequencing method by reading the data descriptions.
For example, in this work it is written that reads that “We took advantage of an intermediate whole-transcriptome amplification (WTA) step that yields full-length cDNAs with cell barcodes (CBs) appended to their 3′ ends.”. So I have to consider it a 3 ‘enrichment, right?
If you have any literature that explain the differences and how to get this information for paper, I would be very happy to study.
• 21 views
Read more here: Source link