Illumina Read Stitching

Illumina Read Stitching


We have implemented the new software provided by Illumina in our mySeq.

The application allows to turn off or on the parametre “Read Stitching”. From the Illumina website, I can understand what a stitched read is. However, I have run many samples with both parameters on and off to compare and see differences and both data generated is very very similar.

Can someone explain to me what is this?

Note: What the software explains when clicked in the interrogation mark:
When enable, paired-end reads that overlap by 10 or more bases are stitched to form a single read.

Based on this info, I have expected a decent difference in the output but as I said, the data is practically the same. ???



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