Exome sequencing one sample on two lanes considered two read groups for marking of PCR duplicates?

Exome sequencing one sample on two lanes considered two read groups for marking of PCR duplicates?

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I’m asking because I don’t know whether to mark different lanes as a different library for the purpose of marking PCR duplicates. My instinct is that different lanes from the same library should still be treated as a single read group, since PCR duplicates could be found across lanes, no?

Thanks in advance.


deduplication


read


groups


exome


sequencing

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