Stacks process_radtags question and scripts

Stacks process_radtags question and scripts

1

How I can modify the quality score to retain more reads in stacks process_radtags? I have paired-end reads from Illumina Next Seq 500.
This is my script:
process_radtags -1 MAGICR1_T.fastq.gz -2 MAGICR2_T.fastq.gz -b Barcode2.txt -o /home/mdominguez/demult_MAGIC_b –renz_1 sphI –renz_2 sau3AI -E phred33 –barcode_dist_2 3 -q -r –disable_rad_check –inline_inline -i gzfastq -t 66

Best regards,

Matias


snp


next-gen

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