Understanding FREQ; Reasons for variant allele frequency to deviate from 50%
What are the reasons to observe variant allele frequency (FREQ) generated on an Illumina DNA sequencing platform around 10-30% or 70-90% for heterozygous variants? As in what are the reasons that this value would deviate from 50%, assuming there is no aneuploidy. Is there any biological mechanism that can explain this or is this just a matter of PCR duplication during sample preparation.
• 25 views
Read more here: Source link