Platanus-allee phasing fail: Error(13): Error, SolveDBG exception!!

Platanus-allee phasing fail: Error(13): Error, SolveDBG exception!!

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Hello there,

I am using Platanus-allee 2.2.2 to generate hybrid assembly of Illumina short reads and PacBio reads. I have got contigs.fa from short reads but phasing step with PacBio reads gives the following error after running for couple of hours

Error(13): Error, SolveDBG exception!! platanus_allee solve_DBG command failed.

Here is the script used for contig assembly and phasing

num_threads=20 
tmp_dir=/path-to-/tem/platanus_allee 
memory_GB=100 
out_prefix=out
minimap2_path=minimap2

# Input reads

PE=trimmed_L1_*.fq
PacBio=pacbio*.fastq.gz

# Contig assembly commands
platanus_allee assemble -t ${num_threads} -tmp ${tmp_dir} -m ${memory_GB} -o ${out_prefix} 
-f $PE 
2>${out_prefix}.assembleLog

# phasing commands 
platanus_allee phase -t ${num_threads} -tmp ${tmp_dir} -o ${out_prefix} -mapper ${minimap2_path} 
-c out_contig.fa 
-IP1 $PE -p $PacBio  
2>${out_prefix}.phaseLog

cat out.phaseLog

platanus_allee version: 2.2.2
platanus_allee phase -t 20 -tmp /path-to-/tem/platanus_allee -o out -mapper minimap2 -c out_contig.fa -IP1 trimmed_L1_1.fq trimmed_L1_2.fq -p /path-to-/pacbio*.fastq.gz 

Error(13): Error, SolveDBG exception!!
platanus_allee solve_DBG command failed.

tail -n 15 path-to-/out_intermediateResults/round1/out.solveDBGLog

dividing nodes based on bubbles ...
dividing scaffolds to adjust bubble-breakpoints...
dividing indonsistent bubble-ends...
pairing bubble nodes...
dividing scaffolds to adjust bubble-breakpoints distiguishing strands ...
NUM_DIVISION = 1832
dividing scaffolds to adjust bubble-breakpoints...
dividing indonsistent bubble-ends...
pairing bubble nodes...
dividing scaffolds to adjust bubble-breakpoints distiguishing strands ...
NUM_DIVISION = 0
TOTAL_NUM_DIVISIONS =1832

dividing erroneous scaffolds based on base-level coverages ...
Segmentation fault (core dumped)

A bug causing the segmentation fault seems issue on Platanus-allee v2.0.0 and earlier

Any suggestion please?


Assembly


genome


next-gen


assembly


software error

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