HTqPCR – Multiple plates for multiple samples: How made a correct pData?

I have a set of seven files from runs on 48 low-throughput qpcr system. So, our runs have 48 max samples. In fact, we have 30 reactions per run. Our file is like this:

File1.csv

1 10-A1-C C9 1 IDH Endogenous Control 12.6929092407226
2 10-A1-C C10 1 IDH Endogenous Control 12.4841232299805
3 10-A1-S C11 2 IDH Endogenous Control 18.4506340026855

30 9-A1-S F2 15 IDH Endogenous Control 17.3977642059326

File2.csv

1 10-A1-C A1 1 UBQ Endogenous Control 11.66
2 10-A1-C A2 1 UBQ Endogenous Control 11.7372970581054
3 10-A1-S A3 2 UBQ Endogenous Control 16.82

30 9-A1-S C6 15 UBQ Endogenous Control 17.3474025726318

File3.csv

1 10-A1-C C9 1 CDPK26 Target 16.9320430755615
2 10-A1-C C10 1 CDPK26 Target 17.0587520599365
3 10-A1-S C11 2 CDPK26 Target 16.5248744964599

30 9-A1-S F2 15 CDPK26 Target 16.9789012908935

File7.csv

1 10-A1-C C9 1 MYB Target 12.5751647949219
2 10-A1-C C10 1 MYB Target 12.959545135498
3 10-A1-S C11 2 MYB Target 10.3745765686035

30 9-A1-S F2 15 MYB Target 12.228588104248

**First** column -> id (1 until 30)

**Second** column -> sample identification (10 = genotype; A1 = region 1; C = symptoms). We have three genotypes (10, 3 and 9); two regions (A1 and A2) and with symptoms and without. So, we have theses RNA samples: 10-A1-C, 10-A1-S, 10-A2-S, 3-A1-C and 9-A1-S.

**Thi …

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