Question 4 2.5 pts After the reaction has begun; you realize that due to a decimal error; you accidentally added the fluorescently labeled di-deoxyribonucleoside triphosphates (ddATP; ddGTP ddCTP ddTTP) at ten times bigher than the desired concentration What do You expect will be the consequence of this mistake? No trace file will be generated because the concentration of the labeled di-deoxyribonucleoside triphosphates has to be perfect The sequence trace file will “fail” towards the beginning of the sequencing read, but improve in quality towards the end ofthe sequencing read The sequence trace file will be generated as normal, it is 5 okto have an overabundance of labeled di-deoxyribonucleoside triphosphates in the sequencing reaction because not all have to be usedup The sequence trace file will be shortened to less than 700reliable bp because it will “fail” towards the end of the sequencing read

Provide a brief summary of the Sanger sequencing method.

a. Frederick Sanger’s sequencing is a chain termination method that is used to generate DNA fragments that terminate at different points using dye-labeled dideoxynucleotides. DNA is separated by electrophoresis on the basis of size. The DNA sequence can be read out on an electropherogram generated by a laser scanner.
b. Frederick Sanger’s sequencing is a chain elongation method that is used to generate DNA fragments that elongate at different points using dye-labeled dideoxynucleotides. DNA is separated by electrophoresis on the basis of size. The DNA sequence can be read out on an electropherogram generated by a laser scanner.
c. Frederick Sanger’s sequencing is a chain termination method that is used to generate DNA fragments that terminate at different points using dye-labeled dideoxynucleotides. DNA is
joined together by electrophoresis on the basis of size. The DNA sequence can be read out on an
electropherogram generated by a laser scanner.
d. Frederick Sanger’s sequencing is a chain termination method that is used to generate DNA fragments that terminate at different points using dye-labeled dideoxynucleotides. DNA is separated by electrophoresis on the basis of size. The DNA sequence can be read out on an electropherogram generated by a magnetic scanner.