DNA and RNA Sequencing – Final (1) | PDF | Dna Sequencing


Chemical Degradation Method (Maxam and Gilbert’s Method)

Developed by  Allan Maxam and Walter Gilbert in 1977Involves the base-specific chemical cleavage of an end-labeled DNA segment to generate a nested set of labeled molecules


The partially cleaved DNA fragment is subjected to five separate chemical reactions, each of which is specific for a particular base or type of baseThe resulting fragments terminate at that specific base followed by high-resolution denaturing gel electrophoresis and detection of the labeled fragments by autoradiography

Reward Your Curiosity

Everything you want to read.

Anytime. Anywhere. Any device.

No Commitment. Cancel anytime.

Footer menu

Get our free apps

Get our free apps

Copyright © 2021 Scribd Inc.

Copyright © 2021 Scribd Inc.


Read more here: Source link