DNA and RNA Sequencing – Final (1) | PDF | Dna Sequencing

 

Chemical Degradation Method (Maxam and Gilbert’s Method)

Developed by  Allan Maxam and Walter Gilbert in 1977Involves the base-specific chemical cleavage of an end-labeled DNA segment to generate a nested set of labeled molecules

 

The partially cleaved DNA fragment is subjected to five separate chemical reactions, each of which is specific for a particular base or type of baseThe resulting fragments terminate at that specific base followed by high-resolution denaturing gel electrophoresis and detection of the labeled fragments by autoradiography

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