Low quality fast5 files
Hi,
I performed direct RNA Seq (with polyA
) to my short sequences (~90 bp
).
However, my fastq_pass are only 20% of the total reads.
I used this quality script to do analysis for the quality score: https://gist.github.com/wdecoster/7cad6080950fa1e3ae3eaeeac4f6ae4d#file-perbasesequencecontentandquality-py
Do you have experience/thoughts for why I am observing such fluctuating qualities through the read body?
I appreciate your feedback.
Thanks!
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