Getting a bowtie2 error when aligning reads

Getting a bowtie2 error when aligning reads

1

I made index from my reference file and run command to align my data the command I used is
bowtie2 -x <index_referance> -1 <single_end_read_path_1.fastq> -s <outputname.sam>
the output im getting is
Error: Must specify at least one read input with -U/-1/-2
(ERR): bowtie2-align exited with value 1

what is wrong in the command I used?


genome


mapping

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