mtDNA-seq by Capture-based hybirdization library method

mtDNA-seq by Capture-based hybirdization library method

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Hi all,

I would like to ask a question.

Recently, we used KAPA hyperprep + hyperChoice (mtDNA probe) to do the mtDNA-seq, but we are facing an issue.

The heteroplasmy level is different with Sanger Sequencing result by a lot.

Could anyone share your experience on this kind of situation?

Thank you.

Sincerely,
Eric


mtDNA

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