STAR- error
Hi.
I’m trying using aligner STAR, with the follow scprit:
/opt/STAR-2.7.9a/bin/Linux_x86_64/STAR
--genomeDir /home/julia.nicoliello/genome_index
--readFilesCommand zcat
--readFilesIn /home/julia.nicoliello/fastq-files/Giselle_S13_L002_R1_001.fastq.gz /home/julia.nicoliello/fastq-files/Giselle_S13_L002_R2_001.fastq.gz
--outFileNamePrefix /home/julia.nicoliello/resultado_star
--outFilterType BySJout
--outSAMunmapped Within
--outSAMtype BAM SortedByCoordinate
--outSAMattrIHstart 0
--outFilterIntronMotifs RemoveNoncanonical
--runThreadN 20
--quantMode TranscriptomeSAM
--outWigType bedGraph
--outWigStrand Stranded
But, I have error 109, with this message:
BAMoutuput.cpp27:BAMoutput: existing because "OUTPUT file" error: could not create output file /home/julia.nicoliello/resultado_star/Giselle/Giselle_STARtmp/BAMsort/19/28
Solution: check that the path exists and you have write permission for this file. Also check unlimit -n and increase it to allow more open files.
I believe the STAR starting, but stop because the error. the paths resultado_star
and resultado_STARtmp
were created, but the process is not finalized.
I have checked the permission and it’s ok for all paths. I don’t know the problem!
Thanks,
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