STAR- error

STAR- error

0

Hi.

I’m trying using aligner STAR, with the follow scprit:

/opt/STAR-2.7.9a/bin/Linux_x86_64/STAR 
  --genomeDir /home/julia.nicoliello/genome_index 
  --readFilesCommand zcat 
  --readFilesIn /home/julia.nicoliello/fastq-files/Giselle_S13_L002_R1_001.fastq.gz /home/julia.nicoliello/fastq-files/Giselle_S13_L002_R2_001.fastq.gz 
  --outFileNamePrefix /home/julia.nicoliello/resultado_star 
  --outFilterType BySJout 
  --outSAMunmapped Within 
  --outSAMtype BAM SortedByCoordinate 
  --outSAMattrIHstart 0 
  --outFilterIntronMotifs RemoveNoncanonical 
  --runThreadN 20 
  --quantMode TranscriptomeSAM 
  --outWigType bedGraph 
  --outWigStrand Stranded

But, I have error 109, with this message:

BAMoutuput.cpp27:BAMoutput: existing because "OUTPUT file" error: could not create output file /home/julia.nicoliello/resultado_star/Giselle/Giselle_STARtmp/BAMsort/19/28
Solution: check that the path exists and you have write permission for this file. Also check unlimit -n and increase it to allow more open files.

I believe the STAR starting, but stop because the error. the paths resultado_star and resultado_STARtmp were created, but the process is not finalized.

I have checked the permission and it’s ok for all paths. I don’t know the problem!

Thanks,


STAR


gene


RNA-Seq

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