ROTLA doesn’t work


I’ve been trying to install Reader of The Lost Arc (ROTLA) package from this github page ( However, every time I try to run any command from this package, I always get the same error. It looks like that:

Traceback (most recent call last):
  File "/home/yablinkubovich/.local/bin/ROTLA", line 11, in <module>
    load_entry_point('ROTLA==0.1.0', 'console_scripts', 'ROTLA')()
  File "/home/yablinkubovich/.local/lib/python2.7/site-packages/click-7.1.2-py2.7.egg/click/", line 829, in __call__
    return self.main(*args, **kwargs)
  File "/home/yablinkubovich/.local/lib/python2.7/site-packages/click-7.1.2-py2.7.egg/click/", line 782, in main
    rv = self.invoke(ctx)
  File "/home/yablinkubovich/.local/lib/python2.7/site-packages/click-7.1.2-py2.7.egg/click/", line 1259, in invoke
    return _process_result(sub_ctx.command.invoke(sub_ctx))
  File "/home/yablinkubovich/.local/lib/python2.7/site-packages/click-7.1.2-py2.7.egg/click/", line 1066, in invoke
    return ctx.invoke(self.callback, **ctx.params)
  File "/home/yablinkubovich/.local/lib/python2.7/site-packages/click-7.1.2-py2.7.egg/click/", line 610, in invoke
    return callback(*args, **kwargs)
  File "/home/yablinkubovich/.local/lib/python2.7/site-packages/ROTLA-0.1.0-py2.7.egg/ROTLA/", line 46, in find_breakpoints
  File "/home/yablinkubovich/.local/lib/python2.7/site-packages/ROTLA-0.1.0-py2.7.egg/ROTLA/", line 39, in __init__
  File "/home/yablinkubovich/.local/lib/python2.7/site-packages/ROTLA-0.1.0-py2.7.egg/ROTLA/", line 408, in execute
    call([self.blat_path, padded_fn, self.output_header + ".read_1.fasta", self.output_header + ".read_1.psl"], stdout=blat_out)
  File "/usr/lib/python2.7/", line 172, in call
    return Popen(*popenargs, **kwargs).wait()
  File "/usr/lib/python2.7/", line 394, in __init__
    errread, errwrite)
  File "/usr/lib/python2.7/", line 1047, in _execute_child
    raise child_exception
OSError: [Errno 2] No such file or directory

I’ve tried to reinstall it, or reinstall BLAT, which is required for this package, but nothing seems to work.
If anybody happened to work with this package before and faced the same problem, please, let me know how you solved it.

Read more here: Source link