Study on plasmid purification and plasmid vector administration into skeletal muscle mediated by electroporation
The purification process to rapidly produce a large of plasmid had been developed and an in vivo electroporation device was made by our own. We evaluated delivery of plasmid vector that encode reporter Enhanced Green Fluorescent Protein (EGFP) into rabbit skeletal muscle. Reporter gene result showed that plasmid DNA transfer in muscle fibers was very efficient by the in vivo electroporation device. According to the result of rabbit intramuscular plasmid injection followed by the electroporation device, the female lambs and pigs were injected with myogenic expression plasmid vectors that encode growth hormone releasing hormone followed by electroporation. The result indicated that the intermuscular injection pM-GHRH plasmid mediated by electroporation on lamb and pig growth does not have the remarkable influence.