Reads detected/”spill over” on introns after using bamCoverage function from deepTools

Reads detected/”spill over” on introns after using bamCoverage function from deepTools

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Hello! We recently used the recent version of bamCoverage from the deepTools suite for our ribo-seq and RNA-seq bam files to generate bigwig files. The conversions ran smoothly, however when we loaded the bigwig files into IGV, we noticed some reads spilling over to some exon-intron junctions when we zoomed into each track. We believe these are not genome misannotations because the non-normalized/non-bigwig bam files don’t show these junction reads. Any advice is appreciated. Thank you!

Best,
Kat


bamCoverage


deepTools


bigwig


intron

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