Error showing Unable to determine input files in Trimmomatic

Error showing Unable to determine input files in Trimmomatic

0

Hello all, I am trying to run trimmomatic on paired fastq file. Can anyone please explain why it is unable to determine the input files?

Fastq files
SO_5492_LR_60A_BR_01_R1.fastq
SO_5492_LR_60A_BR_01_R2.fastq

This is the command I used
Trimmomatic PE -threads 30 -basein SO_5492_LR_60A_BR_01_R1.fastq -baseout trim/SO_5492_LR_60A_BR_01.fq ILLUMINACLIP:./TruSeq3-PE.fa:2:30:10 SLIDINGWINDOW:4:15

Output

TrimmomaticPE: Started with arguments:
-threads 30 -basein SO_5492_LR_60A_BR_01_R1.fastq -baseout trim/SO_5492_LR_60A_BR_01.fq ILLUMINACLIP:./TruSeq3-PE.fa:2:30:10 SLIDINGWINDOW:4:15
Unable to determine input files from: SO_5492_LR_60A_BR_01_R1.fastq


NGS


RnaSeq


Trimmomatic


Fastq

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