counting(quantifying) the expression level of genes from Exom_seq

counting(quantifying) the expression level of genes from Exom_seq

0

Hi everyone,
I am quantifying the expression level of genes from EXOM seq.
I used featureCount function and passed the bam file in the command line as stated in the biostar hand book page number 792 but got errors as shown below in the picture.

GTF=refs/ERCC92.gtf
featureCounts -a $GTF -o counts.txt 23.bam

Probably it could be the GTF file has no exom annotation or something elas……

Thus, wondered if anyone can help me? enter image description here


expression


exom


seq

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