RNA-Seq and qPCR

RNA-Seq and qPCR

0

Greetings!
For a rice gene LOC_Os06g51060

Relative (to control group) Log2FC values obtained by qPCR and RNA-Seq are as follows:

See here:
enter image description here

There is a difference in values as well a TREND. I have quite a few other such genes and this problem has effected them too.

I am concerned about the values not following similar TREND. However, the type (up/down) regulation remains conserved throughout for all my genes. Can anyone please help me with this? is this result acceptable? What can be the cause of it?

Thank you.
Have a great day!

Rgrds,
Akash


trends


RNA-Seq


qPCR-


and


correlation

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