RNA-Seq and qPCR
Greetings!
For a rice gene LOC_Os06g51060
Relative (to control group) Log2FC values obtained by qPCR and RNA-Seq are as follows:
See here:
There is a difference in values as well a TREND. I have quite a few other such genes and this problem has effected them too.
I am concerned about the values not following similar TREND. However, the type (up/down) regulation remains conserved throughout for all my genes. Can anyone please help me with this? is this result acceptable? What can be the cause of it?
Thank you.
Have a great day!
Rgrds,
Akash
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