Which rlog should I use for DESeq2 analysis

Which rlog should I use for DESeq2 analysis

0

@rafaelsolersanblas-22935

Last seen 1 minute ago

Alicante

I have a question regarding the rlog normalization.

I have many samples to compare, with only one factor. A vs Treat, B vs Treat, C vs Treat … So, should I put everything in the same DESeqDataSet object even though the variability between groups is very large (I did the differential expression analysis with the comparisons separated), and then calculate the rlog of all samples?, Or put the different comparisons in different DESeqDataSet objects and extract the rlog of each comparison, and later join the rlogs by EnsemblID?

Thank you!


samples


rlog


DESeq2

• 16 views

Read more here: Source link