Comparing RPKM (calculated from raw counts rnaseq file) between a few genes across 2 experimental conditions with a t-test : bioinformatics

Hello everyone! Very new to analyzing RNAseq data and trying to learn the process behind it. This is completely not my area, but I would like to learn and was hoping someone could give me an explanation to my question. Thank you!

I’d like to know if a few genes I’m interested in are differentials expressed between 2 conditions. I was wondering if anyone could tell me whether it is acceptable to calculate RPKM (or TPM; a normalized read/count metric) for each gene from a raw counts file across each condition (3 replicates per condition) and perform a t-test for each gene (condition 1 v condition 2). Is it always important to run a DEG analysis to see if the genes of interested meet a certain threshold?

Once again, sorry for the really basic question! I’d be forever grateful if you could explain and teach! Thanks!

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