Escherichia coli K-12 substr. MG1655 EC 4.2.1.150



BioCyc ID: RXN-11667

EC Number:
4.2.1.150

Enzymes and Genes:

3-hydroxyacyl-CoA dehydrogenase FadJInferred by computational analysis:
fadJ

multifunctional enoyl-CoA hydratase, 3-hydroxyacyl-CoA epimerase, Δ3cis– Δ2trans-enoyl-CoA isomerase, L-3-hydroxyacyl-CoA dehydrogenaseInferred by computational analysis:
fadB

In Pathway:
oleate β-oxidation

Note that this reaction equation differs from the official Enzyme Commission reaction equations for this EC entry.


The reaction direction shown is in accordance with the Enzyme Commission rules (the physiological direction of the reaction, if known, is indicated by the arrow direction).

Mass balance status: Balanced.


Generic reaction:

a 2,3,4-saturated (3S)-3-hydroxyacyl-CoA ← a (2E)-2-enoyl-CoA + H2O (4.2.1.17)

Enzyme Commission Primary Name:
short-chain-enoyl-CoA hydratase

Enzyme Commission Synonyms:
3-hydroxybutyryl-CoA dehydratase,
crotonase,
crt (gene name)

Standard Gibbs Free Energy (ΔrG‘°):
2.1629639 kcal/mol

Enzyme Commission Summary:

The enzyme from the bacterium
Clostridium acetobutylicum is part of the central fermentation pathway and plays a key role in the production of both acids and solvents. It is specific for short, C4-C6, chain length substrates and exhibits an extremely high turnover number for
crotonyl-CoA. cf.
EC 4.2.1.17, enoyl-CoA hydratase and
EC 4.2.1.74, long-chain-enoyl-CoA hydratase.

Citations:
[Waterson72,
Waterson81,
Boynton96a]

Relationship Links:
BRENDA:EC:4.2.1.150,
ENZYME:EC:4.2.1.150,
IUBMB-ExplorEnz:EC:4.2.1.150

Credits:

Created in MetaCyc 13-Sep-2010 by
Caspi R,
SRI International

Imported
from MetaCyc 14-Mar-2019 by
Caspi R,
SRI International


References

Boynton96a: Boynton ZL, Bennet GN, Rudolph FB (1996). “Cloning, sequencing, and expression of clustered genes encoding beta-hydroxybutyryl-coenzyme A (CoA) dehydrogenase, crotonase, and butyryl-CoA dehydrogenase from Clostridium acetobutylicum ATCC 824.” J Bacteriol 1996;178(11);3015-24. PMID: 8655474

Waterson72: Waterson RM, Castellino FJ, Hass GM, Hill RL (1972). “Purification and characterization of cortonase from Clostridium acetobutylicum.” J Biol Chem 1972;247(16);5266-71. PMID: 5057466

Waterson81: Waterson RM, Conway RS (1981). “Enoyl-CoA hydratases from Clostridium acetobutylicum and Escherichia coli.” Methods Enzymol 1981;71 Pt C;421-30. PMID: 7024731


Report Errors or Provide Feedback

Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 45:D543-550 2017

Page generated by Pathway Tools version 25.0 (software by SRI International) on Thu Nov 25, 2021, BIOCYC14.


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