Mitochondrial mutations in Caenorhabditis elegans show signatures of oxidative damage and an AT-bias


. 2021 Oct 2;219(2):iyab116.


doi: 10.1093/genetics/iyab116.

Affiliations

Item in Clipboard

Gus Waneka et al.


Genetics.


.

Abstract

Rapid mutation rates are typical of mitochondrial genomes (mtDNAs) in animals, but it is not clear why. The difficulty of obtaining measurements of mtDNA mutation that are not biased by natural selection has stymied efforts to distinguish between competing hypotheses about the causes of high mtDNA mutation rates. Several studies which have measured mtDNA mutations in nematodes have yielded small datasets with conflicting conclusions about the relative abundance of different substitution classes (i.e., the mutation spectrum). We therefore leveraged Duplex Sequencing, a high-fidelity DNA sequencing technique, to characterize de novo mtDNA mutations in Caenorhabditis elegans. This approach detected nearly an order of magnitude more mtDNA mutations than documented in any previous nematode mutation study. Despite an existing extreme AT bias in the C. elegans mtDNA (75.6% AT), we found that a significant majority of mutations increase genomic AT content. Compared to some prior studies in nematodes and other animals, the mutation spectrum reported here contains an abundance of CG→AT transversions, supporting the hypothesis that oxidative damage may be a driver of mtDNA mutations in nematodes. Furthermore, we found an excess of G→T and C→T changes on the coding DNA strand relative to the template strand, consistent with increased exposure to oxidative damage. Analysis of the distribution of mutations across the mtDNA revealed significant variation among protein-coding genes and as well as among neighboring nucleotides. This high-resolution view of mitochondrial mutations in C. elegans highlights the value of this system for understanding relationships among oxidative damage, replication error, and mtDNA mutation.


Keywords:

Duplex Sequencing; cytosine deamination; low-frequency variant; metazoan mtDNA; mitochondrial mutation; mutation accumulation; mutation spectra; oxidative damage; oxidized guanine; replication error.

Similar articles

LinkOut – more resources

  • Full Text Sources

  • Miscellaneous

Read more here: Source link