ONT analysis on Ubuntu

ONT analysis on Ubuntu

0

Hello guys,
I am new on bioinformatic analysis of data from ONT (MinION with FLOW-MIN106D). The lab sent me a collection of fast5 files I have to analyze to obtain the metagenomic details (viruses). I have the new machine with on board Ubuntu 21 (Nvidia GE-3080, 96G ram, 24 cores). Nvidia in already working under Ubuntu.
I know the first step to do is the basecalling and the demultiplexing and I would like to use the guppy_basecalling (many manuscripts mention it). I searched for some detailed procedure about the ONT pipeline installation on Ubuntu, but I came very confused: does I need to install the MinKNOW on ubuntu? mioion-nc? What does it mean “live basecalling”? Is the guppy package enough?
Thank you for your help.
Emilio


guppy


calling


minknow


base


demultiplexing


ubuntu

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