About ‘Estimated Number of cells’ in snRNA-seq

About ‘Estimated Number of cells’ in snRNA-seq

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Hi all,

I am analyzing single nucleus RNA-seq data using Seurat.
And I have total four group and 24 samples (Brain region A Control & case and Brain region B Control & case; each n=6).

I wonder what is the appropriate range of the ‘Estimated number of cells’ for analysis.
In our analysis, Each group has slightly different average number of cells. (A Con: 17,000, A case: 9,900, B Con: 10,000, B case: 9,500)
And minimum 5,700 ~ maximum 24,000 in all samples.

So, 1) What is the appropriate range of the number of cells? 2) Is it okay if there is a difference in the number of cells between groups? and 3) What should be done if the number of cells is too high or if the difference in cell number between groups need to be reduced?

Thank you in advance!


snRNA-seq


scRNA-seq


QC

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