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KEGG ENZYME: 1.14.14.86

 December 29, 2021

Entry
EC 1.14.14.86               Enzyme                                 
Name

ent-kaurene monooxygenase;
ent-kaurene oxidase (misleading)

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor

Sysname

ent-kaur-16-ene,[reduced NADPH—hemoprotein reductase]:oxygen oxidoreductase (hydroxylating)

Reaction(IUBMB)
ent-kaur-16-ene + 3 [reduced NADPH—hemoprotein reductase] + 3 O2 = ent-kaur-16-en-19-oate + 3 [oxidized NADPH—hemoprotein reductase] + 4 H2O (overall reaction) [RN:R10066];
(1a) ent-kaur-16-ene + [reduced NADPH—hemoprotein reductase] + O2 = ent-kaur-16-en-19-ol + [oxidized NADPH—hemoprotein reductase] + H2O [RN:R06291];
(1b) ent-kaur-16-en-19-ol + [reduced NADPH—hemoprotein reductase] + O2 = ent-kaur-16-en-19-al + [oxidized NADPH—hemoprotein reductase] + 2 H2O [RN:R06292];
(1c) ent-kaur-16-en-19-al + [reduced NADPH—hemoprotein reductase] + O2 = ent-kaur-16-en-19-oate + [oxidized NADPH—hemoprotein reductase] + H2O [RN:R06293]
Reaction(KEGG) Substrate
ent-kaur-16-ene [CPD:C06090];
[reduced NADPH—hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007];
ent-kaur-16-en-19-ol [CPD:C11872];
ent-kaur-16-en-19-al [CPD:C11873]
Product
ent-kaur-16-en-19-oate [CPD:C11874];
[oxidized NADPH—hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001];
ent-kaur-16-en-19-ol [CPD:C11872];
ent-kaur-16-en-19-al [CPD:C11873]
Comment

A cytochrome P-450 (heme thiolate) protein found in plants. Catalyses three successive oxidations of the 4-methyl group of ent-kaurene giving kaurenoic acid.

History

EC 1.14.14.86 created 2002 as EC 1.14.13.78, transferred 2018 to EC 1.14.14.86

Pathway
ec00904   Diterpenoid biosynthesis
ec01110   Biosynthesis of secondary metabolites
Orthology Genes  » show all
Reference   Authors

Ashman PJ, Mackenzie A, Bramley PM.

  Title

Characterization of ent-kaurene oxidase activity from Gibberella fujikuroi.

  Journal Reference   Authors

Archer C, Ashman PJ, Hedden P, Bowyer JR, Bramley PM.

  Title

Purification of ent-kaurene oxidase from Gibberella fujikuroi and Cucurbita maxima.

  Journal Reference   Authors

Helliwell CA, Poole A, Peacock WJ, Dennis ES.

  Title

Arabidopsis ent-kaurene oxidase catalyzes three steps of gibberellin biosynthesis.

  Journal   Sequence Other DBs
ExPASy – ENZYME nomenclature database:  1.14.14.86

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