p57 suppressed the proliferation of mESCs. (a) The growth curve of control () and p57 knockdown (shp57, ) mESCs. (b, c) EdU staining results of control () and p57 knockdown (shp57, ) mESCs cultured for 48 h. (d) Real-time PCR analyses of pcna, Cyclin A, and Cyclin E expression levels of control () and p57 knockdown (shp57, ) mESCs cultured for 48 h. (e, f) Western blot and related densitometric analysis of PCNA, Cyclin A, and Cyclin E protein levels of control and p57 knockdown (shp57) mESCs cultured for 48 h. (g) The growth curve of control () and p57-overexpressing (p57, ) mESCs. (h, i) EdU staining results of control () and p57-overexpressing (p57, ) mESCs cultured for 48 h. (j) Real-time PCR analyses of pcna, Cyclin A, and Cyclin E expression levels of control () and p57-overexpressing (p57, ) mESCs cultured for 48 h. (k, l) Western blot and related densitometric analysis of PCNA, Cyclin A, and Cyclin E protein levels of control and p57-overexpressing (p57) mESCs cultured for 48 h. μm. , , and .
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