Figure 4 | p57 Suppresses the Pluripotency and Proliferation of Mouse Embryonic Stem Cells by Positively Regulating p53 Activation

p57 suppressed the proliferation of mESCs. (a) The growth curve of control () and p57 knockdown (shp57, ) mESCs. (b, c) EdU staining results of control () and p57 knockdown (shp57, ) mESCs cultured for 48 h. (d) Real-time PCR analyses of pcna, Cyclin A, and Cyclin E expression levels of control () and p57 knockdown (shp57, ) mESCs cultured for 48 h. (e, f) Western blot and related densitometric analysis of PCNA, Cyclin A, and Cyclin E protein levels of control and p57 knockdown (shp57) mESCs cultured for 48 h. (g) The growth curve of control () and p57-overexpressing (p57, ) mESCs. (h, i) EdU staining results of control () and p57-overexpressing (p57, ) mESCs cultured for 48 h. (j) Real-time PCR analyses of pcna, Cyclin A, and Cyclin E expression levels of control () and p57-overexpressing (p57, ) mESCs cultured for 48 h. (k, l) Western blot and related densitometric analysis of PCNA, Cyclin A, and Cyclin E protein levels of control and p57-overexpressing (p57) mESCs cultured for 48 h. μm. , , and .

Read more here: Source link