Bioinformatics HW5.docx – 1 Run a Delta-BLAST with the silkworm insulin protein(P26726 Limit to human proteins in the RefSeq_Protein database How many

1. Run a Delta-BLAST with the silkworm insulin protein (P26726). Limit to human proteins in theRefSeq_Protein database.How many total sequences?

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b. How many human homologs appear to have the insulin domain (irrespective of the e-valuethreshold)?

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c. Now edit search remove the human from the organism selection and change DELTA-BLASTthreshold to 0.005. Keep everything else unchanged. How many records?

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d. Write the name of the organism that has the best e-value?

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2. 2. Run PSI-BLAST with the following sequence. Use the RefSeq database, limit toenterobacteria and change the PSI-BLAST threshold to 0.005.

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