How to perform deconvolution with RPKM values

Hi Xuran,

I tried to apply MuSiC to RNAseq bulk data with RPKM as the input. According to your paper(Discussion), “MuSiC can utilize RPKM if estimates of cell type-specific total RNA abundance can be provided.” I am wondering how I can incorporate cell-type-specific total RNA abundance into your function? Or can I directly use RPKM as the input and use music_prop to do the deconvolution? Does my reference single-cell RNAseq data also requires RPKM as the input?


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