WLQ #5.doc – BIOL/BNFO 541 Wet Lab Quizzes 5 Protocol 3 and 6 3 Agarose gel electrophoresis 6 Cloning 16S rRNA gene PCR fragments into pGEM u00ae-T Easy

BIOL/BNFO 541
Wet Lab Quizzes # 5, Protocol 3 and 6 – March 15, 2019
3. Agarose gel electrophoresis
6. Cloning 16S rRNA gene PCR fragments into pGEM
®
-T Easy plasmid vector
Deadline: March
14
, 2019, Thursday, midnight sharp!
No copying and pasting! Use your own words.
Mario Melchor-Guerra
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1.
What difference should be seen in the migration of the 18S rRNA gene
fragments (Feb 22, 2019) and today’s 16S rRNA gene fragments on agarose
gel electrophoresis? Hint: The diptera primers dip18SF and dip18SR you
used for amplifying the mosquito 18S rRNA gene fragment anneal to
mosquito
Aedes vexans
18S rRNA gene (AM071382.1) sequence at
positions 433-451 and 1716-1695 respectively, while the bacterial primers
16S08F and 16S08R anneal to Nostoc PCC9709 16S rRNA gene
(AF027654) sequence at positions 274-289 and 995-980 respectively. (2/10)

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