HyDrop: droplet-based scATAC-seq and scRNA-seq using dissolvable hydrogel beads

Reviewer #1 (Public Review):

Droplet-based single-cell method development has stalled in the past years. The field has been overtaken by commercial solutions that optimized performance, but at much higher costs and without any possibility for customization. More recently, combinatorial indexing methods (e.g. SPLIT-seq) have gained popularity, requiring no specialized equipment and offering the possibility to massively increase output, but at the cost of reduced sensitivity.

De Rop et al. introduce a flexible microfluidics-based single-cell genomics technology that expands and improves over previously existing custom droplet-based scRNA-seq protocols (inDrops and Drop-seq) in several directions: better data quality, simplified workflow, high-cell recovery, and flexibility towards other single-cell applications, as exemplified by HyDrop-based single-cell ATAC-seq.

This is a much welcome development in the field and one that will hopefully stir the further development and optimization of custom droplet-based single-cell protocols (multi-ome, scChIP, and beyond).


Read the original source

Read more here: Source link