Escherichia coli K-12 substr. MG1655 ackA-pta

BioCyc ID:TU0-8301

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Transcription Unit

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Other Transcription-Units in the Same Operon:
pta (ptap),
ackA (ackAp)

In Pathway:
CreCB Two-Component Signal Transduction System

Summary:

There is a RNase processing sequence in the ackA-pta intergenic region
[De19].

Citations:
[Kakuda94]


Regulation with unknown or unspecified binding site location:


Bound
DNA-binding transcriptional regulator CreB-phosphorylated activates transcription.[
Avison01Inferred from experiment]

Site Summary:
CreB is a response regulator that activates transcription of the ackA-pta operon. In 2001, M. Avison et al. identified a possible binding site for CreB, TTCACCTCAACTTCAC, in the upstream region of the operon [Avison01]. The predicted binding site of CreB has been identified based on similarity to the consensus sequence (acTTCACnnnnacTTCAC) and demonstration of its function based on gene expression analysis [Avison01]. The transcription start site for this operon has not been identified; for this reason, the binding site has no central position. This response regulator requires one direct repeat binding site of 18 bp to activate transcription of the ackA-pta operon.


Regulation of Translation:

Citations:
[Miyakoshi19,
De19]

Position of mRNA Binding Site Relative to Translation Start Site (bp):
-2 – 8

Sequence:


References

Avison01: Avison MB, Horton RE, Walsh TR, Bennett PM (2001). “Escherichia coli CreBC is a global regulator of gene expression that responds to growth in minimal media.” J Biol Chem 276(29);26955-61. PMID: 11350954

De19: De Mets F, Van Melderen L, Gottesman S (2019). “Regulation of acetate metabolism and coordination with the TCA cycle via a processed small RNA.” Proc Natl Acad Sci U S A 116(3);1043-1052. PMID: 30591570

ElMansi21: El-Mansi M, Phue JN, Shiloach J (2021). “Expression of the ace operon in Escherichia coli is triggered in response to growth rate-dependent flux-signal of ATP.” FEMS Microbiol Lett 368(2). PMID: 33417680

Kakuda94: Kakuda H, Hosono K, Shiroishi K, Ichihara S (1994). “Identification and characterization of the ackA (acetate kinase A)-pta (phosphotransacetylase) operon and complementation analysis of acetate utilization by an ackA-pta deletion mutant of Escherichia coli.” J Biochem 116(4);916-22. PMID: 7883769

Miyakoshi19: Miyakoshi M, Matera G, Maki K, Sone Y, Vogel J (2019). “Functional expansion of a TCA cycle operon mRNA by a 3′ end-derived small RNA.” Nucleic Acids Res 47(4);2075-2088. PMID: 30541135


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Please cite the following article in publications resulting from the use of EcoCyc: Frontiers in Microbiology 2021

Page generated by Pathway Tools version 26.0 (software by SRI International) on Wed Apr 20, 2022, BIOCYC17.


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