Solved 4. Now that we designed the primers, and run the PCR

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4. Now that we designed the primers, and run the PCR reaction, we are ready to subclone. First, we will use the restriction enzyme EcoRI to create sticky ends in the plasmid and the PCR product, then ligate them together. To check the reaction, we will digest the final PCR/Plasmid DNA using various enzymes. Using the maps below and supplemental Restriction Gel exercise answer the following questions.
For example, lane 1 (EcoRI) would cut the PCR product back out of the plasmid, resulting in and bands. 1. Eco RI 2. BamHI 3. XbaI and EcoRI 4. NheI 5. Xbal and Nhe I 6. BamHI, EcoRI, XbaI
PCR product

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