Is low N in-vivo research a blind spot for RNA-seq?

My thoughts on this have very much not changed since you asked a similar question two years ago: Am I crazy, or are most published RNA-seq studies vastly underpowered?

Both your proposed explanations are in play – but I would say probably that the first is more important. An example of this you don’t really mention is that if you do a sample of 5 humans, you have 5 different organisms: they have have different genetics, have had different life experiences, different things for breakfast this morning, etc. Where as 5 replicates of a cell line are (or at least started off) genetically identical. They all share the same life history etc. Its much better to regard 5 replicates of a cell line as being more like 5 samples from the same person as samples from 5 different people.


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