DRAGEN .bcl conversion error due to improper custom p5 oligos

DRAGEN .bcl conversion error due to improper custom p5 oligos

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I am working on a custom library preparation method and I designed my p5 oligos incorrectly. To be specific, I used the reverse complement of the correct p5 index sequence. As a result my fastq files aren’t demultiplexing properly. Ideally, this should be an easy problem to fix, just switch the sequences in the sample sheet to match what I actually made, but for some reason DRAGEN’s bcl conversion software refuses to run it and says there’s an error with the sample sheet. I’m assuming it’s because the index sequences don’t match what it’s supposed to be, but I’m well aware of that discrepancy and would like it if the sheet could just run. Illumina has been less than helpful on this front. Has anyone else ever had this issue and found a work around?


NextSeq2000


bcl


bcl2fastq


samplesheet


DRAGEN

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