PRIME PubMed | 16S to 23S rRNA spacer fragment length polymorphism of Salmonella enterica at subspecies and serotype levels

TY – JOUR
T1 – 16S to 23S rRNA spacer fragment length polymorphism of Salmonella enterica at subspecies and serotype levels.
AU – Christensen,H,
AU – Møller,P L,
AU – Vogensen,F K,
AU – Olsen,J E,
PY – 2000/8/17/pubmed
PY – 2001/2/28/medline
PY – 2000/8/17/entrez
SP – 130
EP – 6
JF – Journal of applied microbiology
JO – J Appl Microbiol
VL – 89
IS – 1
N2 – The variation in the lengths of the internal transcribed spacer (ITS) between 16S and 23S rRNA genes of 101 strains representing 58 serotypes of Salmonella enterica (used for Salm. choleraesuis) subsp. enterica (I), salamae (II), arizonae (IIIa), diarizonae (IIIb), houtenae (IV) and indica (VI) was used for typing by PCR amplification. Ten fragment lengths were observed by denaturing polyacrylamide gel electrophoresis on an automatic DNA sequencer resulting in 21 unique fragment patterns. Ten out of the 58 serotypes showed specific patterns but 48 serotypes were not fully differentiated. More than one ITS pattern was observed in seven serotypes. Five of the 21 fragment patterns contained representatives of more than one subspecies. Under non-denaturing electrophoresis conditions, serotype specificity was obtained but precise ITS fragment length determination was not possible. DNA sequence comparison between ITSs of individual rrn operons is needed to further interpret ITS diversity within Salm. enterica at serotype and subspecies levels.
SN – 1364-5072
UR – brain.unboundmedicine.com/medline/citation/10945789/16S_to_23S_rRNA_spacer_fragment_length_polymorphism_of_Salmonella_enterica_at_subspecies_and_serotype_levels_
L2 – onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=1364-5072&date=2000&volume=89&issue=1&spage=130
DB – PRIME
DP – Unbound Medicine
ER –