Remodeling of H3K9me3 during the pluripotent to totipotent-like state transition


. 2022 Dec 25;S2213-6711(22)00595-1.


doi: 10.1016/j.stemcr.2022.12.006.


Online ahead of print.

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Hu Li et al.


Stem Cell Reports.


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Abstract

Multiple chromatin modifiers associated with H3K9me3 play important roles in the transition from embryonic stem cells to 2-cell (2C)-like cells. However, it remains elusive how H3K9me3 is remodeled and its association with totipotency. Here, we integrated transcriptome and H3K9me3 profiles to conduct a detailed comparison of 2C embryos and 2C-like cells. Globally, H3K9me3 is highly preserved and H3K9me3 dynamics within the gene locus is not associated with gene expression change during 2C-like transition. Promoter-deposited H3K9me3 plays non-repressive roles in the activation of genes during 2C-like transition. In contrast, transposable elements, residing in the nearby regions of up-regulated genes, undergo extensive elimination of H3K9me3 and are tended to be induced in 2C-like transitions. Furthermore, a large fraction of trophoblast stem cell-specific enhancers undergo loss of H3K9me3 exclusively in MERVL+/Zscan4+ cells. Our study therefore reveals the unique H3K9me3 profiles of 2C-like cells, facilitating the further exploration of totipotency.


Keywords:

H3K9me3; pluripotency; totipotency; transposable elements (TEs).

Conflict of interest statement

Conflict of interests The authors declare no competing interests.

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