HTSeq error processing GFF file

HTSeq error processing GFF file

0

Hello,

I am trying to run HTSeq but it tells me that I have a problem in my GFF and GTF file, how can I fix this?

enrique@L:~prueba_guess$ htseq-count -f bam SRR214880.bam -s no -i ID -r pos -t exon  GCF_902167145.1_Zm-B73-REFERENCE-NAM-5.0_genomic.gff > SRR214880.txt

100000 GFF lines processed.
200000 GFF lines processed.
300000 GFF lines processed.
400000 GFF lines processed.
500000 GFF lines processed.
600000 GFF lines processed.
700000 GFF lines processed.
800000 GFF lines processed.
900000 GFF lines processed.

Error processing GFF file (line 997531 of file GCF_902167145.1_Zm-B73-REFERENCE-NAM-5.0_genomic.gff):
  Strand must be'+', '-', or '.'.
  [Exception type: ValueError, raised in _HTSeq.pyx:72]

My GFF file:

enter image description here


HTSeq


GFF


GTF

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